A1. Feasibility study.

A1. Feasibility study. (3)

Selection and characterization of specific wastes from REYDESA to be bioleached (origin, shape, suitable pretreatments, etc.) and study on possibilities to use available waste streams from nearby other industries for nutrients solution.

A1.3. Principal scheme for microbiological treatment of PCBs of WEEE.

16 October 2020 A1. Feasibility study. 45 Views

Figure 3a — Crushed Printed Circuit Boards (PCBs) of WEEE in the Reydesa facility in Legutio, Spain, 17 July 2018.
Photo by A. Menert.

Figure 3b — Milled and densitometrically separated PCBs of WEEE from Reydesa, magnification 10X.
Photo by S. Kuuse.

Bioleached e-waste (Estonian origin), magnification 10X.
Photo by S. Kuuse

A1.2. Study of microbial biodegradation potential of indigenous species of ARGCON5 with bioleaching cultivation experiments in microcosms with OxiTop AN measuring system.

16 October 2020 A1. Feasibility study. 34 Views

Figure 2a — The flasks of OxiTop AN measuring system with PCBs of WEEE in growth medium inoculated with ARGCON5 and with growth medium and inoculum, 100th day of experiment. The flask with PCBs of WEEE is remarkably darker.
Photo by A. Menert.

Figure 2b — The flasks of OxiTop AN measuring system with PCBs of WEEE in growth medium inoculated with ARGCON5 adapted to e-waste, with growth medium and inoculum only and with PCBs of WEEE in growth medium (without inoculum), 10th day of experiment. In the flask with all components added, gas bubbles indicate degradation of organic matter.
Photo by A. Menert.

The flasks of OxiTop AN measuring system with PCBs of WEEE in distilled water. The liquid phase is crystal clear without haze typical to bacterial growth.
Photo by A. Menert.

Figure 2d — The flasks of OxiTop AN measuring system are maintained in thermostated shaker at t=37°C during the whole experiment.
Photo by S. Sipp Kulli.

Figure 2e — Pressure increase recorded on data logger indicates the start of methane production.
Photo by S. Sipp Kulli.

A1.1. Preparation of inoculum for the bioleaching cultivation experiments.

16 October 2020 A1. Feasibility study. 47 Views

Figure 1a — Before placing into the bioleaching experiment, the Inoculum CELMS No EEUT ARGCON5 is pre-cultivated in test tubes in a thermostated shaker at t=37°C.
Photo by S. Sipp Kulli.

After pre-cultivation the remarkable haze in test tubes with inoculum demonstrates the growth of bacteria.
Photo by S. Sipp Kulli.

Figure 1c — The BiotaTec coworkers of Kärt Ukkivi, MSc and Anne Menert, PhD are examining the quality of inoculum CELMS No EEUT ARGCON5. The Inoculum has grown up and is ready to be added to the non-metallic fraction of PCBs of WEEE.
Photo by S. Sipp Kulli.