B2.3. Set-up and Operation

14 Juuni 2021 B2. Pilot building and operation. 50 Views

Before the adaptation of the microorganisms, a series of processes to adapt the laboratory media to the work procedures in bioleaching were carried out:

 

- The development of a safety and health protocol has been developed

- Sterilization techniques for the media have been studied

- Study of the microorganisms that could be present in the reaction conditions that can compete with the Acidithiobacillus ferroxidans.

- Non-metallic part of PCB pre-treated with saturated NaCl and further washed

- Test equipment performance during scaling up (250 ml - 1 L - 10 L - 50 L)

Non-metallic part of PCB pre-treated with saturated NaCl and further washed

 

Adaptation and scaling up process of Acidithiobacillus ferroxidans with the non-metallic fraction obtained after mechanical treatment of PCBs:

 

The process consists in 6 steps:

- Step 1: Vials inoculation

- Step 2: Adaptation to 250 ml

- Step 3: Adaptation to PCB in 250 ml

- Step 4: Adaptation to PCB in 1 L

- Step 5: Adaptation to PCB in 10 L

- Step 6: Adaptation to PCB in 50 L

Steps 1-3 from adaptation and scaling up process.

 

Steps 4-6 from adaptation and scaling up process.