A1. Feasibility study.

A1. Feasibility study. (3)

Selection and characterization of specific wastes from REYDESA to be bioleached (origin, shape, suitable pretreatments, etc.) and study on possibilities to use available waste streams from nearby other industries for nutrients solution.

A1.1. Preparation of inoculum for the bioleaching cultivation experiments.

16 Oktoober 2020 A1. Feasibility study. 159 Views
Figure 1a
Before placing into the bioleaching experiment, the Inoculum CELMS No EEUT ARGCON5 is pre-cultivated in test tubes in a thermostated shaker at t=37°C.
Photo by S. Sipp Kulli.

 

After pre-cultivation the remarkable haze in test tubes with inoculum demonstrates the growth of bacteria.
Photo by S. Sipp Kulli.

 

Figure 1c
The BiotaTec coworkers of Kärt Ukkivi, MSc and Anne Menert, PhD are examining the quality of inoculum CELMS No EEUT ARGCON5. The Inoculum has grown up and is ready to be added to the non-metallic fraction of PCBs of WEEE.
Photo by S. Sipp Kulli.

A1.2. Study of microbial biodegradation potential of indigenous species of ARGCON5 with bioleaching cultivation experiments in microcosms with OxiTop AN measuring system.

16 Oktoober 2020 A1. Feasibility study. 166 Views
Figure 2a
The flasks of OxiTop AN measuring system with PCBs of WEEE in growth medium inoculated with ARGCON5 and with growth medium and inoculum, 100th day of experiment. The flask with PCBs of WEEE is remarkably darker.
Photo by A. Menert.

 

Figure 2b
The flasks of OxiTop AN measuring system with PCBs of WEEE in growth medium inoculated with ARGCON5 adapted to e-waste, with growth medium and inoculum only and with PCBs of WEEE in growth medium (without inoculum), 10th day of experiment. In the flask with all components added, gas bubbles indicate degradation of organic matter.
Photo by A. Menert.

 

Figure 2a
The flasks of OxiTop AN measuring system with PCBs of WEEE in distilled water. The liquid phase is crystal clear without haze typical to bacterial growth.
Photo by A. Menert.

A1.3. Principal scheme for microbiological treatment of PCBs of WEEE.

16 Oktoober 2020 A1. Feasibility study. 178 Views
Figure 3a
Crushed Printed Circuit Boards (PCBs) of WEEE in the Reydesa facility in Legutio, Spain, 17 July 2018.
Photo by A. Menert.

 

Figure 3b
Milled and densitometrically separated PCBs of WEEE from Reydesa, magnification 10X.
Photo by S. Kuuse.

 

Figure 3c
Bioleached e-waste (Estonian origin), magnification 10X.
Photo by S. Kuuse